Post by alanwalker on Jan 13, 2018 9:23:01 GMT -5
Hi,
at the moment I`m preparing for my first 2D TLC to separate phospholipids and there`s something, I dont understand, although it`s probably very obvious, but I dont get it.
If I can applicate only one sample per round on the silica gel and if I -because of this- cant applicate standards at the same time on the same gel, how can I identify the spots in the end? Or do I have to use an extra gel for each sample (unknown and standards) and develop it in the same chamber or -if no space is left- in another one during the same time? But this would mean a huge wastage of gels... I`m a bit confused.
And another question - to separate lipids (phospholipids and cholesterol) of a fluid - is it really necessary to do a 2D TLC? Or does it perhaps also work with 1D?
Please help.
Thanks!
I didn't find the right solution from the Internet.
References: www.chromforum.org/viewtopic.php?t=20127
Brand promo video
at the moment I`m preparing for my first 2D TLC to separate phospholipids and there`s something, I dont understand, although it`s probably very obvious, but I dont get it.
If I can applicate only one sample per round on the silica gel and if I -because of this- cant applicate standards at the same time on the same gel, how can I identify the spots in the end? Or do I have to use an extra gel for each sample (unknown and standards) and develop it in the same chamber or -if no space is left- in another one during the same time? But this would mean a huge wastage of gels... I`m a bit confused.
And another question - to separate lipids (phospholipids and cholesterol) of a fluid - is it really necessary to do a 2D TLC? Or does it perhaps also work with 1D?
Please help.
Thanks!
I didn't find the right solution from the Internet.
References: www.chromforum.org/viewtopic.php?t=20127
Brand promo video